Omics · study · 2026
Single cell RNA sequencing of hippocampus and hypothalamus in AD mice with fructose-induced MetS and DHA and NR supplementation
Listed in NCBI GEO
Alzheimer’s disease (AD) is a pervasive neurodegenerative disorder with hallmarks of beta-amyloid (Aβ) plaques and neurofibrillary tangles.
Description
In light of the suboptimal benefit of Aβ clearing drugs to patients, the association between metabolic disorders and AD provides an alternative angle to understand the role of metabolic dysfunction in AD pathogenesis and the potential of modulating metabolism for improved therapeutic development.
We investigated the effects of metabolic and nutritional modulators, namely, high fructose consumption as a potential risk, and docosahexaenoic acid (DHA) and nicotinamide riboside (NR) as potentially beneficial, on cell type specific transcriptomic responses in the hippocampus and hypothalamus of an AD mouse model. Fructose-induced metabolic syndrome increased expression of complement component 3 in ependymal cells and inflammatory genes in microglia, which were normalized by DHA and NR.
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NR and DHA led to reversals of Aβ signatures across cell types and targeted different aspects of microglial reactivity between AD and metabolically challenged AD. Our study supports the impact of metabolic regulation on neuroinflammation and provides molecular support for utilizing metabolic modulators for AD intervention.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE292nnn/GSE292341 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE292341 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1238200 ncbi.nlm.nih.gov/bioproject/PRJNA1238200 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Disease 75% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE292341 | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |