miRNA expression profiling of visceral adipose tissue-derived extracellular vesicles in nicotine-treated ApoE-/- mice

Mice were fed either a high-fat diet (HFD) or a high-fat diet combined with nicotine administration (HFD+Ni). Epididymal white adipose tissue (eWAT) was used as a representative visceral fat depot for EV isolation.

High-throughput small RNA sequencing was performed to characterize the miRNA cargo of VAT-EVs and to identify differentially expressed miRNAs between the two groups. The resulting dataset provides a resource for investigating nicotine-associated alterations in EV-mediated miRNA signaling in the context of metabolic and inflammatory processes.