Omics · study · 2026
Effect of HSD and HFD on cardiac function in Drosophila
Listed in NCBI GEO
Type 2 diabetes is a multifactorial disease that has a pandemic development worldwide.
Description
Associated cardiac dysfunctions are affecting one-third of the patients. Drosophila has proven to be a valuable model to study cardiac function in physiological and physio-pathological contexts.
Indeed, adult flies challenged on rich diets (high sugar or high fat) developed cardiac dysfunctions similar to diabetic cardiomyopathies. In order to better characterize the molecular events leading to these cardiac diseases, we performed an RNA-sequencing on poly(A) extracted from 10 days old female flies reared on ND (Normal Diet), HSD (High Sugar Diet) or HFD (High Fat Diet). The differential expression of genes was evaluated for each rich diet versus normal diet.
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Genes exhibiting a fold change ≥ 1.5 were considered as good candidates. Gene Ontology analysis for cellular localization showed a significant enrichment in extracellular component. This was supported by the presence of a signal peptide in 27% in HSD and 44% in HFD of the candidate genes.
These results suggest that putative new secreted proteins have been identified, putting forward the role of the heart as a secretory organ in hyperglycemia and hyperlipidemia. We functionally validated some interesting candidates by specific cardiac-KO and evaluated the different cardiac phenotypes by high-speed live imaging on undissected flies. We focused on the fit gene, encoding a previously identified satiety hormone and characterized its paracrine as well as its systemic cardiac function in the adult.
Our results illustrate a yet unknown cardiac function for the Fit hormone and suggest it as a new fly cardiokine.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE235nnn/GSE235313 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE235313 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA985686 ncbi.nlm.nih.gov/bioproject/PRJNA985686 ↗
project · from NCBI GEO
- PubMed 42275457 pubmed.ncbi.nlm.nih.gov/42275457 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Drosophila melanogaster · Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE235313 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[anatomy].local:anatomy:heart | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:imaging | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:7227 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |