Reduced Representation Bisulfite Sequencing (RRBS) of hippocampus from Mtr-cKO and wild-type mice

Genomic DNA was extracted from hippocampus, quantified, and quality-checked prior to RRBS library preparation using the Diagenode Premium RRBS kit. Bisulfite conversion efficiency was evaluated using methylated and unmethylated spike-in controls.

Single-end 75 bp sequencing was performed on an Illumina NextSeq 550 system. Raw FASTQ files and processed CpG methylation tables are provided. Differential methylation analysis was performed using methylKit (R), including filtering, normalization, CpG calling, DMR identification, and genome annotation.