Omics · study · 2026
TSPAN8 a plasticity marker is transcriptionally regulated through DUSP19 BRD4 crosstalk in Prostate Cancer cells
Listed in NCBI GEO
To identify upstream regulators of TSPAN8 transcription, we screened a library of 298 phosphatases using a GFP reporter under the control of the TSPAN8 promoter in PC3 cells.
Description
DUSP19 emerged as the strongest negative regulator. Unexpectedly, DUSP19-mediated regulation of TSPAN8 was independent of its canonical JNK pathway: pharmacological JNK inhibition with SP600125 did not affect TSPAN8 expression, and pJNK/JNK levels were unchanged by siDUSP19.
RNA-seq combined with promoter binding-site analysis identified BRD4 as the candidate effector linking DUSP19 to TSPAN8 transcription, and pharmacological BRD4 inhibition (AZD5153) reversed the siDUSP19-induced TSPAN8 upregulation at both mRNA and protein levels. Additionally, TSPAN8 accumulation is significantly higher in biopsies SYNAPTOPHYSIN+.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE341nnn/GSE341968 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE341968 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1504923 ncbi.nlm.nih.gov/bioproject/PRJNA1504923 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE341968 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |