Omics · study · 2026
An Immunocompetent Transplantable Mouse Model of Basal Cell Carcinoma
Listed in NCBI GEO
Basal cell carcinoma (BCC) is the most common type of cancer, yet mouse models of BCC are limited.
Description
Existing BCC mouse models such as the Ptch1+/--K14-CreERT2p53fl/fl-tumor (GEM-BCC) model rely on genetic engineering. These models are essential for studying BCC carcinogenesis but are complicated to integrate into preclinical treatment evaluation studies due to the spontaneous growth of multiple tumors with heterogenous growth.
This study describes the establishment of an immunocompetent transplantable BCC (Tp-BCC) mouse model suitable for preclinical treatment evaluation. By in vivo passaging GEM-BCC tumor cells, we were able to establish a Tp-BCC model. We compared microstructural features, tumor microenvironment and transcriptional profile of the Tp-BCC model with its parental GEM-BCC model using histology, flow cytometry and RNA sequencing.
Read the rest (2 more)
Tp-BCC tumors displayed synchronized growth and shortened time until tumor formation compared to GEM-BCC tumors. Tp-BCC tumors showed micro-nodular histological structure and had a tumor immune microenvironment composition similar to the GEM-BCC tumors. Hedgehog signaling pathway-related genes were similarly expressed except for Igf2.
The transcriptional profile of Tp-BCC model was less variable than the GEM-BCC model. This study demonstrates that the Tp-BCC model resembles the clinically relevant GEM-BCC model while providing an immunocompetent high-throughput model for evaluating treatments of BCC.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311891 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE311891 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1371893 ncbi.nlm.nih.gov/bioproject/PRJNA1371893 ↗
project · from NCBI GEO
- PubMed 42733821 pubmed.ncbi.nlm.nih.gov/42733821 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE311891 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |