Isoform-resolved transcriptome in patients with inflammatory bowel disease

Numerous omics studies have been published to understand IBD pathogenesis and identify potential targets for therapeutics. Despite the high amount RNA-seq data from short reads sequencing platforms produced to help preclinical and clinical research in IBD, such studies fail to capture the full-length mRNAs and various isoforms from alternative splicing events.

Thus, the transcriptome associated with IBD has been understudied. The IsoSeq system of PacBio Sequel platforms enables sequence large number of long transcripts, and the corresponding IsoSeq pipeline and additional bioinformatics tools can identify the full-length transcripts and various isoforms. Here, we generate isoform-resolved transcriptome in IBD patients by performing long-read RNA sequencing on samples from four IBD patients and two healthy controls and integrate the full-length transcripts data with short-read RNA sequencing data from 99 IBD patients and controls.