Omics · study · 2026
Low frequency parasitized cells in bone marrow alter myelopoiesis following immunization with the live attenuated Leishmania major Cen-/- vaccines
Listed in NCBI GEO
We have developed a live-attenuated Leishmania major strain lacking the Centrin gene (LmCen-/-) as a vaccine candidate got leishmaniasis, which has shown safety and efficacy in pre-clinical studies.
Description
Studies in leishmanization models have shown that persistent infection is necessary for maintaining protective immunity. Here, we evaluated the presence of latent parasites in cells from the bone marrow and their effect on the immune landscape.
Dual-scRNAseq analysis identified parasitized cells in the bone marrow 28 days post-infection in mice following intradermal inoculation with virulent LmWT or LmCen-/- strains. Flow cytometry analysis showed that inoculation with LmCen-/- parasites resulted in elevated myelopoiesis compared to LmWT infection. Cell communication analysis revealed that elevated granulin-sort3 signaling in LmCen-/- infection could be mediating the myelopoiesis.
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Monocytes isolated from the bone marrow of LmCen-/- and LmWT infected mice killed virulent LmWT parasites in co-culture experiments suggesting the induction of trained immunity in the bone marrow.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE346nnn/GSE346567 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE346567 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1525916 ncbi.nlm.nih.gov/bioproject/PRJNA1525916 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE346567 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |