Transcriptome profiling of embryonic stem cells (mESCs) derived from B6 mouse, primitive neural stem cells (NSCs) derived from mESCs, muscle fibers, fat cells, spleen cells and thymus using RNA-sequencing.

Therefore, cancer cells can differentiate along different lineages. Upon differentiation, cancer cells will lose their neural stemness and tumorigenicity.

Cancer cells, embrynonic stem cells and neural stem cells are immune-privileged cells and are capable of immune evasion. We used transcriptome profiling to analyze the difference in trasncriptomes and particularly, transcription of immune related genes in a few cell types representing undifferentiated and differentiated states, because immunigenicity is the key for effective anti-tumor immunity and this relies on expression of immune related genes.