Omics · study · 2026
Pertussis toxin-induced lymphocytosis enhances anti-tumor immune environments and improves the anti-PD1 treatment response in a squamous cell carcinoma model.
Listed in NCBI GEO
Elevation of the neutrophil-to-lymphocyte ratio (NLR) in cancer patients predicts a worse prognosis, and NLR is a good discriminator in response to anti-tumor immune checkpoint inhibitors.
Description
However, the therapeutic potential of NLR normalization to enhance the efficacy of anti-tumor immunotherapy has not been investigated. In this study, we chose pertussis toxin (PTX) as an agent to induce lymphocytosis and lower NLR.
PTX decreased NLR in squamous cell carcinoma (SCC)-tumor-bearing mice and shifted the tumors from low to high immune scores. PTX-induced elevation of CXCL9 led to lymphocytosis and increased immune cell infiltration in tumor tissues. The combination treatment with PTX and anti-PD1 antibody significantly suppressed in vivo SCC tumor growth, compared to anti-PD1 treatment alone.
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The combination treatment induced a larger proportion of T cells in the tumor microenvironment, and these findings were associated with an increase in terminally differentiated CD8 T cells and their augmented Ki-67 expression. In addition, tumor-specific IFN-γ production was augmented in animals treated with anti-PD1 antibody and combination treatments, but not with PTX alone. In summary, PTX can induce high lymphocyte infiltration into tumor tissue, leading to the NLR normalization and a favorable immune environment for immune checkpoint inhibitors.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311831 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE311831 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1370977 ncbi.nlm.nih.gov/bioproject/PRJNA1370977 ↗
project · from NCBI GEO
- PubMed 42209867 pubmed.ncbi.nlm.nih.gov/42209867 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE311831 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |