Omics · study · 2026
Regulation of endothelial cell chromatin availability and transcription factor activity in arterial-venous specification - Murine Fucci Retinal EC ATACseq
Listed in NCBI GEO
Arterial-venous specification of endothelial cells during vascular development requires coordination between intracellular signaling, cell cycle state, and transcription factor activity.
Description
However, the intrinsic regulatory mechanisms that govern these processes are poorly understood. To investigate this, we assessed endothelial chromatin accessibility during vascular development.
Murine postnatal day (P)6 and P15 retinal endothelial cells were analyzed by single cell Assay for Transposase Accessible Chromatin (ATAC) sequencing, revealing heterogeneous accessibility of chromatin across an arterial-venous continuum. Enhancer regulatory network analysis predicted transcription factors with high activity, including Sox17. Transcription factors with differential arterial-venous activity showed dual activator and repressor functions, with many regulated by cell cycle-dependent chromatin accessibility.
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We validated SOX17 function in human endothelial cells, identifying that SOX17 inhibits proliferation and promotes arterial gene expression. Our findings suggest that dual roles of key endothelial transcription factors are regulated by chromatin accessibility in a cell cycle- and subtype-specific manner to control arterial-venous specification.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE293nnn/GSE293306 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE293306 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1243982 ncbi.nlm.nih.gov/bioproject/PRJNA1243982 ↗
project · from NCBI GEO
- PubMed 42093597 pubmed.ncbi.nlm.nih.gov/42093597 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Genome binding/occupancy profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Sequencing 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE293306 | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:genome-binding-occupancy-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |