Omics · study · 2026
RNA-seq of Smc3-GFP flox/flox mouse embryonic stem cells with conditional knockout
Listed in NCBI GEO
To investigate the functional consequences in the absence of Smc3 in mouse embryonic stem cells (mESCs), we treated the Smc3 GFP flox/flox mESCs with 4-OHT to deplete the Smc3 alleles, and then performed RNA-seq on two independent Smc3 flox/flox GFP ESC lines before and after Cre-mediated excision.
Description
RNA-seq analysis revealed dysregulation of key genes governing pluripotency and lineage commitment, highlighting the indispensable role of cohesin in preserving the transcriptional identity of embryonic stem cells.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE325nnn/GSE325947 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE325947 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1442691 ncbi.nlm.nih.gov/bioproject/PRJNA1442691 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE325947 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |