Omics · study · 2026
Targeting DHFR to suppress 1-C metabolism and enhance radiation sensitivity in NRF2-active head and neck squamous cell carcinoma
Listed in NCBI GEO
NRF2 hyperactivity is associated with radiation resistance in head and neck squamous cell carcinoma (HNSCC).
Description
We evaluated the dihydrofolate reductase (DHFR) inhibitors pyrimethamine and its potent analog, WCDD115, as radiosensitizing agents through disruption of one-carbon metabolism.Preclinical studies used NRF2-competent and deficient HNSCC models, clonogenic assays, transcriptomics, metabolomics, pharmacokinetics/toxicity testing, and syngeneic tumor grafts to assess WCDD115 as a novel radiosensitizing agent.
Correlative analysis included mass spectrometry, western blotting, and pathway analysis. WCDD115 enhanced radiation-induced DNA damage and suppressed one-carbon metabolism and nucleotide biosynthesis.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE345nnn/GSE345515 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE345515 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1521030 ncbi.nlm.nih.gov/bioproject/PRJNA1521030 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Cancer 65% · Mass spectrometry 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE345515 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:mass-spectrometry | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |