Role of Ikaros in Mature IgM+ B-cells: Studies of IkZnF1-/- and IkZnF4-/- mutant mice [RNA-seq]

The C2H2 zinc finger is the most prevalent DNA-binding motif in the mammalian proteome, with DNA-binding domains usually containing more tandem fingers than are needed for stable sequence-specific DNA recognition. To examine the reason for the frequent presence of multiple zinc fingers, we recently generated mice lacking finger 1 or finger 4 of the 4-finger DNA-binding domain of Ikaros.

Each mutant strain exhibited a specific subset of the phenotypes observed with Ikaros null mice, and revealed that different subsets of fingers within multi-finger transcription factors can regulate distinct target genes and biological functions. We here study the effect of these mutants on mature B-cells with transcriptome profiling of sorted IgM+ B-cells from BM of wt and the two ZnF mutants (RNA-Seq).