Omics · study · 2026
The whole-genome duplication is a barrier for iPSCs reprogramming from mouse fibroblasts
Listed in NCBI GEO
Whole-genome duplication (WGD) -induced tetraploidization represents a pivotal genetic event widely investigated in the contexts of tumorigenesis, developmental processes, and evolutionary biology, whereas its functional consequences for somatic cell reprogramming have yet to be elucidated.
Description
Here we show that mouse embryonic fibroblasts (MEFs) undergo spontaneous WGD in vitro. However, the resulting tetraploid cells are refractory to iPSCs reprogramming.
Mechanistically, the tetraploidization of MEFs is p38-dependent. Pretreatment with p38 inhibitor SB203580 suppresses cytokinesis failure-induced binucleation and significantly enhances reprogramming efficiency. We further identify the elevated p53 accumulation in binucleated cells as the primary barrier to reprogramming, as its depletion rescues iPSCs formation in tetraploid cells.
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These findings reveal the whole-genome duplication exerts distinct regulatory control over cell fate transition.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE295nnn/GSE295779 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE295779 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1256352 ncbi.nlm.nih.gov/bioproject/PRJNA1256352 ↗
project · from NCBI GEO
- PubMed 41888895 pubmed.ncbi.nlm.nih.gov/41888895 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE295779 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |