Omics · study · 2026
Decoding the Protein Composition of Whole Nucleosomes with Knic
Listed in NCBI GEO
Histone post-translational modifications (PTMs) controls the gene expression profile via transcriptional regulation．Aberrant histone PTMs chromatin structures lead to dysregulated gene expression and cancer progression.
Description
Nicotinic acid (vitamin B3 or vitamin PP), one of the thirteen essential vitamins, has a wide range of uses, helping to function digestive system, skin and nervous system. Here we show nicotinic acid (NA) induces histone and non-histone lysine nicotinylation, and identify 17 nicotinylation sites on core histones in HeLa cells.
Also, NA-derived nicotinylation of histone lysine residues is an epigenetic modification that regulates gene transcription via altering the chromatin accessibility by generating nicotinyl-CoA. Knic inactivates oncogene PPFIA1 transcription by inhibiting its combination with transcription factors HOXB9, and PPFIA1 was found as an important role in promoting cells spreading on the extracellular matrix and is required for migration and invasion in human breast tumor cells 1,2.
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However, the role and the molecular mechanisms of PPFIA1 control hepatocellularcarcinoma (HCC) initiation and progression are poorly understood. Here we demonstrate PPFIA1 promotes proliferation, migration, invasion and tumorigenesis of HCC in vivo and in vitro. It is worth noting that the expression of PPFIA1 is upregulated in multiple human carcinomas, including HCC.
Our study also suggests that PPFIA1 is a promising potential target of NA for the future treatment strategies of HCC. Here, we show that administration of NA inhibits liver cancer metastasis through suppressing tumor gene PPFIA1 transcription by inducing histone Knic.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE229nnn/GSE229807 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE229807 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA956317 ncbi.nlm.nih.gov/bioproject/PRJNA956317 ↗
project · from NCBI GEO
Topics
- Stated by source
- Genome binding/occupancy profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE229807 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:genome-binding-occupancy-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |