Omics · study · 2026
Type I IFN Drives ILC2 to ILC1 Plasticity in Radiation-Induced Lung Injury
Listed in NCBI GEO
Radiation-induced lung injury (RILI) is a common dose-limiting toxicity after radiotherapy for lung cancer, especially after ablative radiotherapy such as Stereotactic Body Radiation Therapy (SBRT).
Description
However, the immunological mechanisms driving its pathogenesis remain poorly understood. Here we show that innate lymphoid cells (ILCs) orchestrate early immune remodeling after ablative irradiation.
Flow cytometry and cytokine profiling revealed a radiation-induced shift from lung-resident ILC2s toward pro-inflammatory ILC1s. Single cell RNA sequencing analysis of lung ILC populations after radiation suggested that type I interferon (IFN) promoted radiation-induced ILC2 to ILC1 plasticity. In addition, IFN-β stimulation of ILC2s in vitro led to increased expression of T-bet and IFN-γ.
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Inhibition of type I IFN signaling attenuated lung damage in irradiated mice, suggesting a potential therapeutic strategy for mitigating RILI. Our findings highlight a previously unrecognized role of type I IFN in radiation-induced ILC plasticity that contributes to RILI pathogenesis. Importantly, this work provides a framework for developing immune-targeted interventions to reduce normal tissue toxicity during radiotherapy.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE309nnn/GSE309235 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE309235 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1334384 ncbi.nlm.nih.gov/bioproject/PRJNA1334384 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE309235 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |