Data · dataset · 2026
Unravelling key regulators of cell fate changes during epithelial-mesenchymal transition
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.17034/32640423.v1
Tumour metastasis is considered as a significant challenge to patient survival, meanwhile epithelial to mesenchymal transition (EMT) as a key driver of tumour aggressiveness.
Description
Understanding the mechanisms underlying EMT regulation is crucial for developing effective strategies to prevent further cancer cell metastasis. While many transcription factors and histone regulators have been identified as promoters of EMT, the understanding of regulators that can prevent mesenchymal transition and maintain epithelial cell identity is limited.
In our study, we aimed to uncover novel transcription factors involved in EMT regulation using chromatin profiling through ATAC-seq (Assay for Transposase-Accessible Chromatin sequencing). Through this approach, we identified a previously uncharacterized transcription factor – PITX1 (Paired Like Homeodomain 1) which displayed a unique pattern of activity during the global reduction of chromatin accessibility induced by TGF-β in human mammary epithelial cells.
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We also investigated the expression and activity level of PITX1 across various cancers. We further found that PITX1 showed significantly higher activity and expression in both epithelial carcinoma cells and EMT - low breast cancer patients (Figure 3.5). Furthermore, we conducted in vitro experiments to confirm the role of PITX1 in maintaining epithelial cell identity and preventing mesenchymal-like transitions.
Our results demonstrated that the depletion of PITX1 enhanced the expression levels of specific genes involved in promoting EMT, supporting its function in preserving epithelial identity. Our analysis revealed a decline in promoter site accessibility during EMT progression. Notably, PITX1 exhibits higher expression in epithelial breast cancer cells, with its knockdown leading to increased mesenchymal gene expression and chromatin decompaction.
The impact of PITX1 on EMT and the tumour microenvironment underscores its significance in cancer biology. By unravelling the mechanisms through which PITX1 and other similar regulators preserve epithelial characteristics, we can develop targeted therapies aimed at preventing metastasis by maintaining epithelial cell identity.<br>
Links
Where it is published
- DOI doi.org/10.17034/32640423.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Cancer · Cancer · Cancer · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Epigenetics · Epigenetics · Epigenetics · Medicine & Health · Medicine & Health · Medicine & Health
- Inferred from text
- Sequencing 75%
Provenance · 3 source records, 17 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32640423 | 7 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32640423 | 7 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32640423 | 7 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[disease].local:disease:cancer | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['cancer'] |
| concepts[disease].local:disease:cancer | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['cancer'] |
| concepts[disease].local:disease:cancer | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['cancer'] |
| concepts[field].anzsrc:field:310504 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['epigenetics'] |
| concepts[field].anzsrc:field:310504 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['epigenetics'] |
| concepts[field].anzsrc:field:310504 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['epigenetics'] |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |