Excel · study · 2026
The role of the NADPH oxidase complex in the defense against Aspergillus nidulans clinical isolates
Listed in NCBI GEO
Epidemiological data indicate that Aspergillus nidulans shows a unique association with patients with chronic granulomatous disease (CGD), accounting for the prevalence of fungal infections in this population.
Description
The mechanisms underlying this association remain poorly understood. We characterized 24 clinical A. nidulans isolates through genomic sequencing, phenotypic profiling, and host-pathogen interaction studies using wild-type and gp91phox⁻/⁻ (CGD) murine macrophages, complemented by in vivo apocynin-treated murine models.
Pangenome analysis revealed a predominantly closed genome (82.2% core) with geographic population structure and strain-specific biosynthetic gene clusters. Phenotypic characterization showed substantial strain-to-strain variability in stress responses, antifungal susceptibility, and cell wall architecture. While CGD macrophages exhibited reduced ROS and increased conidial survival, only a subset of A. nidulans isolates demonstrated virulence comparable to A. fumigatus.
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Transcriptional profiling identified strain-specific immune modulation, with one isolate exhibiting distinct immune-evasion signatures. Apocynin treatment paradoxically protected against A. fumigatus (70% to 40% lethality) but not A. nidulans, reflecting differential inflammatory responses. Our findings challenge the notion that A. nidulans possesses unique virulence attributes predisposing it to CGD.
The epidemiological association likely reflects strain-specific determinants, species misidentification within the A. nidulans complex, and host immune context. Virulence appears to be a distributed property rather than a species-level trait, emphasizing the need for individualized isolate characterization.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE342nnn/GSE342884 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE342884 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1457744 ncbi.nlm.nih.gov/bioproject/PRJNA1457744 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Disease 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE342884 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |