Endothelial cell cycle inhibition enables blood vessel maturation to normalize the tumor vasculature [bulkRNA-seq]

We identified cell cycle state-specific gene signatures by conducting bulk RNA sequencing on FACS-isolated murine Fucci2-expressing normal ECs (NECs) from healthy mammary fat pads in early and late G1.

Differential gene expression analysis comparing early vs. late G1 NECs revealed the top significantly enriched genes in each phase that were used to generate cell cycle module scores that were applied to all cells in our scRNAseq datasets obtained for tumor, normal and tumor treated with CDK4/6i.