Supplementary file 1_Albuminuria, kidney function, and automatically quantified retinal vascular phenotypes in hospitalized adults with type 2 diabetes.docx

Joint, complete-case, alternative-equation, restricted-range, categorical, spline, and tighter-temporal-window analyses were treated as supportive or exploratory.</p>Results<p>The final cohort included 658 eyes from 337 patients; the median absolute laboratory-report-to-image interval was 1 day (IQR, 0–1), and 97.9% of eye records were within 3 days. In the primary separate core models, neither UACR nor 2021 CKD-EPI eGFR was associated with any of the eight vascular outcomes after FDR correction (minimum q = 0.090).

UACR showed a nominal association with arterial density (q = 0.097), and eGFR showed nominal associations with overall, arterial, and 3-mm foveal vessel density. Exploratory complete-case analyses yielded stronger eGFR signals: in the 431-eye/220-patient joint complete-case cohort, the eGFR-only model in the same cohort was FDR-significant for all eight outcomes, and seven remained significant after mutual adjustment with UACR.

Alternative-equation and restricted-range analyses also yielded eGFR-related associations, whereas same-day analyses were imprecise and null. In a post hoc smoking-adjusted joint model, however, none of the eight eGFR associations remained significant after FDR correction.</p>Conclusions<p>The primary analyses did not demonstrate FDR-significant associations of either kidney marker with the retinal vascular phenotypes.

Exploratory complete-case and sensitivity analyses suggested a model-dependent eGFR-related pattern, whereas UACR remained largely null. These cross-sectional findings are hypothesis-generating and require independent longitudinal validation.</p>