Nanostraw Electroporation for Temporal RNA Sampling from Living 2D and 3D Cell Culture Systems - RNA sequencing data

This limits the ability to track transcriptomic changes within the same cell population and poses challenges for experiments with scarce primary samples. To address this, we developed a minimally perturbative, unbiased RNA sampling approach — nano-electroextraction (NEE) — which uses hollow nanostraw membranes with mild electroporation to extract intracellular RNA without compromising cell viability or gene expression.