Archive · study · 2026
Spatial Multi-Omics Landscape of Colorectal Cancer Macro- and Micrometastases
Listed in NCBI GEO
Colorectal cancer (CRC) metastases often recur due to minimal residual disease (MRD) and micrometastases that persist after therapy.
Description
Here, we analyzed 341,328 spatially resolved spots from 37 tumors, including liver (CLiM) and lung (CLuM) metastases, and primary CRC from 11 patients. Phylogenetic analysis of copy number variations revealed heterogeneous tumor evolution, with early dissemination and quiescence in liver micrometastases (CLiMi).
Spatial analysis identified 10 conserved and 7 distinct metaprograms, highlighting stromal interactions in CLiM and CLuM, with macrophages enriched in CLiM and lymphocytes in CLuM. Micrometastases showed strong immunosuppressive signature and increased T cell exhaustion. Notably, we identified a CLiMi-specific six-gene signature associated with MRD status, disease-free survival, and chemotherapy resistance across multiple independent cohorts.
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These findings elucidate the spatial evolutionary landscape of CRC metastases and provide tissue-based spatially validated biomarkers for surveillance and therapeutic targeting.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE294nnn/GSE294385 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE294385 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1249293 ncbi.nlm.nih.gov/bioproject/PRJNA1249293 ↗
project · from NCBI GEO
- PubMed 42425074 pubmed.ncbi.nlm.nih.gov/42425074 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Homo sapiens · Other
- From keywords
- Life Sciences
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE294385 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |