Omics · study · 2026
Comprehensive analysis of the effects of mitomycin C on human conjunctival fibroblasts
Listed in NCBI GEO
RNA-Seq analysis was conducted to investigate the effects of mitomycin C (MMC) on human conjunctival fibroblasts (HConFs).
Description
Significant changes in gene expression were observed at 3 hours, 3 days, and 14 days after MMC treatment, with 322, 4,231, and 5,095 genes significantly altered compared to controls, respectively. Enrichment analysis revealed a notable upregulation of genes involved in apoptosis and the p53 pathway, as well as a significant downregulation of genes associated with cell proliferation such as those involved in the G2M checkpoint and E2F targets.
Furthermore, the expression of genes related to the senescence-associated secretory phenotype, including FGF2, CCL2, and CCL7, was also increased, suggesting that MMC induces cellular senescence in HConFs as indicated by RNA-Seq analysis.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE306nnn/GSE306051 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE306051 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1309022 ncbi.nlm.nih.gov/bioproject/PRJNA1309022 ↗
project · from NCBI GEO
- PubMed 42625400 pubmed.ncbi.nlm.nih.gov/42625400 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE306051 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |