Compartmentalisation of m6A (N6-methyladenosine) methylated RNA in somatic or axonal subcellular locations is dependent on Nerve Growth Factor (NGF) state in sympathetic neurons [Axon-TRAP-seq]

Cells were then subjected to NGF starvation for 18 hours followed by 1 hour of restimulation. Following a TRAP protocol, translating RNA was collected from either cell bodies (CB) or axons (Ax) from these neurons for RNA sequencing.

Here, we find a strong compartmentalisation effect. We also find that only a few RNA are translated in restimulated distal axons within 1 hour compared with control condition axons.