Gene expression profiling study by RNA-sequencing in lung cancer

Despite advances in our understanding of lung cancer oncogenesis, the mechanism by which lung cancer becomes aggressive and leads to patient death is still poorly characterized. We found that lncRNA RAMS11 is significantly upregulated in lung cancer patients compared to healthy patients.

To investigate the downstream transcriptional effects of RAMS11 expression, we conducted transcriptome sequencing (RNA-seq) in two NSCLC cell lines (A549 and H838) following RAMS11 knockdown or a non-targeting control. Differential expression analysis identified 331 genes regulated by RAMS11. Using the Drug-Gene Interaction Database (DGIdb) we identified 3 and 7 clinically actionable genes that were consistently down- and up-regulated across both cell line models, respectively.