Omics · study · 2026
Ly6C monocyte subsets transcriptome analysis from control and diabetes mice
Listed in NCBI GEO
Monocytes (MC) can be classified as inflammatory and anti-inflammatory subsets.
Description
Inflammatory MC subset polarization is a hallmark of systemic and tissue inflammatory in diabetes. The underlying molecular mechanism remains unclear.
This study aims to explore molecule targets and signaling which determinate immunological features in MC subsets. C57/BL6 and diabetes mice (n=5) were fed a normal chow diet. C57/BL6 mice on rodent chaw were used as CT, C57/BL6 mice treated with streptozotocin (STZ, intraperitoneally, 50mg/kg per day for 5 successive days at the age of 8 weeks) as T1DM, and db/db mice with homozygous mutation of leptin receptor as T2DM.
Read the rest (1 more)
Animals were sacrificed at 20-22 weeks of age for blood collection after euthanization. Blood Ly6Chigh and Ly6Clow MC subsets from control and diabetes mice were isolated by flow cytometry sorting and subjected for bulk high-throughput RNA-sequencing.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE305nnn/GSE305525 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE305525 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1306385 ncbi.nlm.nih.gov/bioproject/PRJNA1306385 ↗
project · from NCBI GEO
- PubMed 42183265 pubmed.ncbi.nlm.nih.gov/42183265 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Computed tomography 50%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE305525 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:ct | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (50%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |