Omics · study · 2026
Endogenous Nitroalkene Exploits Dependence on Autophagy-Lysosome Pathway in PARPi-Resistant Triple Negative Breast Cancer
Listed in NCBI GEO
Our published data show that electrophilic nitroalkenes (NFAs) selectively kill triple negative breast cancer (TNBC) cells over benign breast epithelial cells.
Description
Preliminary data suggests that NFAs are more cytotoxic to TNBC pre-treated with PARP inhibitors (PARPi). In order to better understand PARPi resistance and the impact of NFAs on PARPi-resistant cells, we generated PARPi-resistance in clinically relevant TNBC cell lines, ones that have mutations in the BRCA1 gene.
We subsequently performed an RNA-seq analysis to (1) identify changes in gene expression that are associated with PARPi-resistance and (2) identify pathways in PARPi-resistant TNBC that are targeted by NFA-treatment. Preliminary results show that NFA treatment induces significant transcriptomic changes in mutBRCA1 TNBC cells.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE297nnn/GSE297522 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE297522 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1265122 ncbi.nlm.nih.gov/bioproject/PRJNA1265122 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE297522 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |