hnRNPC engages mature RNAs during metaphase: RNA-seq and fCLIP in proTAME-arrested HeLa/HeLa S3 cells

Heterogeneous nuclear ribonucleoprotein C (hnRNPC) is a ubiquitous RBP that normally binds uridine-rich sequences to influence pre-mRNA processing and stability. Here we investigated the role of hnRNPC in mitotic cells by profiling both global RNA abundance and hnRNPC–RNA interactions during metaphase arrest.

To this end, we combined bulk RNA-seq with fluorescent crosslinking and immunoprecipitation (fCLIP) in proTAME-arrested HeLa S3 cells. This dataset enables the characterization of hnRNPC binding to mature RNAs during mitosis, the evaluation of biochemical fractionation (low-density vs high-density complexes), and the assessment of binding site resolution across distinct RNase digestion conditions. Together, the study provides a resource for exploring how hnRNPC contributes to RNA stability and gene expression control during cell division