Excel · study · 2026
Consequences of reactive astrogliosis in vivo III
Listed in NCBI GEO
To investigate the role of LZK kinase activity in the astrocyte transcriptome, we locally injected AAV2/5 GfaABC1D-Myc-LZK, the kinase-dead mutant AAV2/5 GfaABC1D-Myc-LZK (K195A), or the viral baseline control AAV2/5 GfaABC1D-tdTomato into the dorsal striatum of C57BL/6NTac mice.
Description
AAV2/5 GfaABC1D-RiboTag was co-injected to enable purification and sequencing of actively translated astrocyte mRNAs. Three weeks after AAV injection, striata were homogenized, and RNA was purified from either cleared lysates, representing the input fraction, or HA-immunoprecipitated astrocyte-specific ribosome-associated RNA, representing the IP fraction.
These analyses revealed that LZK kinase activity is required to induce transcriptomic changes associated with cell migration, apoptosis, proliferation, and synaptic transmission.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336013 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336013 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1480568 ncbi.nlm.nih.gov/bioproject/PRJNA1480568 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Sequencing 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE336013 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |