Omics · study · 2026
RNA sequencing of mouse palatal epithelial cells following TGF-β3 treatment or Tgfb3 knockdown
Listed in NCBI GEO
Epithelial-mesenchymal transition (EMT) of medial edge epithelial cells is a critical mechanism underlying embryonic palatal fusion, driven by transforming growth factor beta 3 (TGF-β3).
Description
However, its regulatory interplay with downstream cascades remains poorly understood. To elucidate cellular reprogramming mechanisms during palatogenesis, embryonic day 15 C57BL6/J mouse palatal epithelial cells were isolated to eliminate mesenchymal interference.
To evaluate transcriptomic shifts, next-generation RNA sequencing was performed on cells cultured in three groups: a control group (no treatment), a group treated with 1 ng/mL of recombinant TGF-β3 to induce EMT, and a group with siRNA transfection Tgfb3 knockdown. This dataset provides the global gene expression profiles underlying TGF-β3-mediated palatal fusion, revealing EMT as the main pathway.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE343nnn/GSE343560 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE343560 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1511867 ncbi.nlm.nih.gov/bioproject/PRJNA1511867 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE343560 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |