miRNA Expression and Clinical Data in the Urinary Bladder Cancer and Control Cohort

MiRCURY LNA RT Kit (Qiagen, USA, Cat.No./ID: 339340) was used for reverse transcription and polyadenylation of miRNA to cDNA. Predesigned miRCURY LNA miRNA Custom PCR Panel YCA34464 (Qiagen, USA, Cat. No. / ID: 339322) were used for quantification of preselected miRNAs.

RT-qPCR data using the miRNA panel was processed with Applied Biosystems® ViiA™ 7 Real-Time PCR according to manufacturer’s protocol. An automatic threshold and baseline were used for all the miRNAs to record the CT value. The clinical data were collected retrospectively from Pauls Stradins Clinical University Hospital and Riga East University Hospital patient medical records.