MPO/HOCl upregulates gene expression of mitophagy in murine macrophages

In this study, we treated murine macrophage cell line with different concentrations of HOCl to clarify the regulatory role of MPO on inflammatory environment. Results showed that HOCl could induce the upregulation of autophagy-related genes and extensive changes on mitochondria-related gene expression.

These results were validated by other experiments, like RT-qPCR and WB. And we also observed that HOCl could induce colocalization of autophagosomes and mitochondria in confocal images, showing mitophagy. These results were consistent with in vivo experiments showing that macrophage mitophagy was MPO-dependent.