Excel · study · 2026
RNA-seq of paired epicardial adipose tissue and left ventricular myocardium from 40 human donor hearts across a range of body mass index
Listed in NCBI GEO
Bulk RNA sequencing of paired epicardial adipose tissue (EAT) and left ventricular basal anterior myocardium from 40 de-identified human donor hearts declined for transplantation (Washington Regional Transplant Community; George Washington University IRB determined not human-subjects research).
Description
Donors span body mass index 16-49 kg/m2 and anterior epicardial fat coverage 46-100%. Ribo-depleted, stranded total RNA libraries (TruSeq Stranded Total RNA, Ribo-Zero Gold) were sequenced as 2x101 bp paired-end reads on an Illumina NovaSeq 6000.
The resource supports analyses of adiposity-, hypertension-, coronary artery disease- and sex-associated transcriptional programs in the two tissues and of EAT-myocardium molecular coupling. Targeted metabolomics and lipidomics for the same donors are deposited separately at Metabolomics Workbench.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE334nnn/GSE334029 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE334029 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1473616 ncbi.nlm.nih.gov/bioproject/PRJNA1473616 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Heart 65% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE334029 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[anatomy].local:anatomy:heart | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |