Excel · study · 2026
Intranasal Artesunate Ameliorates Hippocampal LFP Abnormalities and Cellular Apoptosis in APP/PS1 Mice via a Mechanism Involving GHRH/PI3K/Akt Signaling
Listed in NCBI GEO
Alzheimer’s disease (AD) is characterized by neuronal damage and abnormal electroencephalogram (EEG) activity.
Description
While Artesunate (ART) exhibits neuroprotective properties, its specific effects on local field potentials (LFPs) and the underlying molecular mechanisms remain unclear. In this study, we investigated the therapeutic effects and mechanisms of ART in AD models.
In vivo electrophysiological recordings in freely moving APP/PS1 mice revealed that ART attenuated the “EEG slowing” phenotype by rebalancing the LFP power spectral distribution. Furthermore, ART modulated theta-gamma phase-amplitude coupling (PAC) and ameliorated specific physiological properties of sharp-wave ripples (SWRs). Concurrently, morphological and ultrastructural analyses demonstrated that ART attenuated neuronal apoptosis and mitigated pathological alterations in myelin sheaths, synapses, and mitochondria in the hippocampus.
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Mechanistically, transcriptome analysis identified growth hormone-releasing hormone (GHRH) as a primary target of ART intervention. In Aβ1-42-treated HT22 cells, ART improved cell viability. Pharmacological inhibition assays indicated that the anti-apoptotic effects of ART are mediated via the GHRH-PI3K-Akt signaling axis.
Activation of this pathway induced the inhibitory phosphorylation of GSK-3β and promoted the nuclear translocation of NF-κB p65 to upregulate Bcl-2 expression, which collectively inhibited the caspase cascade. Collectively, these findings demonstrate that ART ameliorates abnormal EEG activity and neuronal damage in AD models through the GHRH-PI3K-Akt signaling axis, providing a mechanistic basis for its therapeutic application in AD.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE346nnn/GSE346759 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE346759 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1526580 ncbi.nlm.nih.gov/bioproject/PRJNA1526580 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Disease 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE346759 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |