RNA sequencing of Follicular Cytotoxic CD8+ T Cells from Trypanosoma cruzi-infected mice

Tfc cells emerged transiently in both organs with similar kinetics and antigen specificity, yet segregated primarily according to tissue of analysis at the transcriptional level. Splenic Tfc cells exhibited enhanced glycolytic and mTORC1-associated signatures, increased mitochondrial mass, and stronger cytotoxic and B cell helper functions, promoting immunoglobulin production and plasmablast death. In contrast, Tfc cells from lymph node preferentially displayed memory-associated features, including higher CD127 and CD122 expression, increased IL-2 production, and preferential persistence during chronic infection.

Together, these findings indicate that Tfc populations from spleen and lymph nodes are characterized by distinct transcriptional, metabolic, and functional states.