Omics · study · 2026
Faecalibacterium prausnitzii induces an anti-inflammatory response and a metabolic reprogramming in human monocytes
Listed in NCBI GEO
We isolated immune cells from human blood of patients with IBD and non-inflamed controls.
Description
We stimulated them with F. prausnitzii EXL01 strain, Cadaverin or lipopolysaccharide (LPS) from Escherichia coli and performed Legendplex, ELISA, flow cytometry, RNA-sequencing (RNAseq), and Seahorse analysis. F. prausnitzii EXL01 strain induced the production of IL-10 in CD14+ monocytes from systemic circulation and intestinal tissue of IBD patients and non-inflamed controls in a direct and dose-dependent manner, without inducing a pro-inflammatory response as compared to LPS stimulation.
RNAseq analysis confirmed these results and revealed that F. prausnitzii EXL01 strain differentially affects cell energy metabolism compared to LPS. The anti-inflammatory response induced by F. prausnitzii in monocytes was dependent on mitochondrial activity. F. prausnitzii induces an anti-inflammatory response and rewires energy metabolism in human monocytes, which might explain its beneficial impact on intestinal inflammation and human health in general.
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These results provide new insight into the mechanisms underlying the anti-inflammatory effects of F. prausnitzii and are crucial for a better understanding of its potential use in the treatment of IBD.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE285nnn/GSE285037 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE285037 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1201079 ncbi.nlm.nih.gov/bioproject/PRJNA1201079 ↗
project · from NCBI GEO
- PubMed 41553310 pubmed.ncbi.nlm.nih.gov/41553310 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE285037 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |