RNA profiling of wild-type and hnRNP A1/A2B1-knockout splenic B cells

This process requires the germinal center (GC) reaction of B cells, which generates high-affinity plasma cells. The development of germinal center B (GCB) cells is tightly regulated at multiple levels, including transcriptional, post-transcriptional, and epigenetic mechanisms.

Among these, alternative splicing (AS), a key post-transcriptional regulatory process, plays an essential role in mediating B cell development and differentiation. The heterogeneous nuclear ribonucleoprotein (hnRNP) family is a major group of regulators of alternative splicing. hnRNP A1 and hnRNP A2B1 are the two most abundantly expressed RNA-binding proteins in this family, yet their functions in B cells remain unclear.