Text · study · 2026
Genome-wide CRISPR screening on cytotoxic T lymphocytes mediated cell killing independent of Casp3, Mlkl, and Bid
Listed in NCBI GEO
To gain insight into cytotoxic lymphocyte–mediated, Casp3, necroptosis, and Mitochondrial outer membrane permeabilization (MOMP) independent cancer cell death, we leveraged the a CTL–αCD3–P815 killing system and performed a genome-wide CRISPR screen.
Description
Cas9 expressing and Casp3-/-Mlkl-/-Bid-/- mouse mastocytoma P815 cells were first transduced with Brie gRNA library and subjected to cytotoxic T lymphocytes-mediated selection.
Surviving cells were collected and sequenced to identify genes/gRNAs enriched following selection.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336305 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336305 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1481243 ncbi.nlm.nih.gov/bioproject/PRJNA1481243 ↗
project · from NCBI GEO
Topics
- Stated by source
- Mus musculus · Other
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE336305 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |