Dual host-pathogen RNA sequencing of wild-type and EZH2-deficient Hoxb8-derived macrophages during Mycobacterium tuberculosis infection

To define the host and bacterial transcriptional responses associated with EZH2 loss, we performed dual host-pathogen RNA sequencing of parental Cas9-expressing Hoxb8-derived macrophages and CRISPR-generated EZH2-deficient macrophages under uninfected conditions and at 3 days after infection with Mtb Erdman smyc'::mCherry.

Host and pathogen transcriptomes were quantified to identify macrophage programs associated with bacterial restriction and bacterial stress-adaptation responses.