Dataset for "Cell surface remodeling caused by the loss of the flippase subunit TMEM30A in immune cells"

Using a live‑cell lipid reporter, membrane order probe, and surface proteome mapping, we show that TMEM30A‑knockout cells display robust PS externalization accompanied by faster lateral diffusion of membrane constituents and decreased plasma membrane order. Surface proteome reorganization includes increased abundance of tetraspanins and CD47. Further, TMEM30A loss triggers glycocalyx remodeling via ADAM10‑dependent shedding that removes major transmembrane mucins, including CD43 and CD162.

Together, these data reveal a coordinated reorganization of lipids, glycans, and proteins upon TMEM30A loss, suggesting mechanistic links between flippase dysfunction and increased plasma membrane dynamics and potential sensitization to immune therapy. Furthermore, our study provides an integrated surfaceome framework that might shed light on the relationship between TMEM30A expression and clinical outcomes in cancer.</p><p dir="ltr"><b>Data usage</b></p><p dir="ltr">Researchers are welcome to use the data contained in the dataset for any projects.

Please cite this item upon use or when published. We encourage reuse using the same CC BY 4.0 License.</p><p dir="ltr"><b>Data Content</b></p><p dir="ltr">Excel files for graphs</p><p dir="ltr"><b>Software to open files</b></p><p dir="ltr">.csv - Microsoft excel</p>