Data supporting an auditable sparse-evidence workflow for phage receptor-binding-protein prioritization and Gp17-mediated magnetic capture of Yersinia enterocolitica O:3

Individual well-level values are unavailable and were not reconstructed. V3's description of "36 technical wells" and its single-assay interpretation are superseded for the current analysis. Separate binding fits give apparent parameters of 41.21, 46.37 and 45.67 micrograms/mL: 44.41 +/- 2.80 micrograms/mL (mean +/- between-experiment sample SD).

This mass-concentration parameter concerns the tagged preparation, not intrinsic molar affinity. Historical 44.38 +/- 2.98 (estimate +/- SE; R-squared 0.9904) remains an earlier analysis; its SE must not be relabelled SD. Binding uses the unrounded mean of recorded negative values.

Specificity uses matched experiment rows and each group's negative-control mean, with paired two-sided tests and Holm correction. Small n limits assumptions. The corrected outputs, source workbooks and analysis code were already published in v1.1.0, doi.org/10.5281/zenodo.23156488, under extension_v04/04_ELISA, extension_v04/01_source_snapshot/ELISA and extension_v04/analysis/analyze_elisa.py.

The added JoB_reanalysis_correction_20261006.zip contains 09_JoB_reanalysis_20261006: corrected derived tables, summary, ELISA figure, source hashes and figure crosswalk. It does not replace code. Historical software v1.0.0 remains at doi.org/10.5281/zenodo.22625899.

Software and third-party licences remain separate. The retrospective evidence extensions distinguish 827 accession records from 744 unique sequences and examine identity, missing evidence and receptor/structure input requirements. They do not establish independent predictive accuracy, complete external prediction or cross-target generalization.

The functional case concerns tagged φYeO3-12 Gp17, distinct from prior AP5-derived Gp17 magnetic-capture work. The historical 87.2% count index lacks recoverable volume/dilution normalization and is not absolute recovery. Food-extract observations are not a surface-inoculation challenge or validated detection limit.

Current JoB Figure 4 (ELISA) corresponds to historical V3 Figure 3. Consult the new correction README and figure crosswalk before combining versions. Original source and image-provenance limitations remain applicable.