Omics · study · 2026
RNAseq data of mouse B16-F10 melanoma cell lines after siEzh2 knockdown or GSK126 methyl-transferase inhibitor or DZNep treatment
Listed in NCBI GEO
Enhancer of zeste homolog 2 (EZH2) plays critical roles in the progression of many malignancies including melanoma.
Description
The most well-known EZH2 mechanism is regulating gene expression through catalysing tri-methylation of histone H3 at Lys 27 (H3K27me3). However, EZH2 also have methylation-independent activities that limit the therapeutic potential of EZH2 methyl-transferase inhibitors.
To investigate the methyl-transferase independent role of EZH2 in melanoma, we performed global gene expression analysis of B16-F10 cells treated with methyl-transferase inhibitor GSK126 or siEzh2 knockdown or the EZH2 degrader DZNep.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE189nnn/GSE189489 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE189489 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA783267 ncbi.nlm.nih.gov/bioproject/PRJNA783267 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE189489 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |