Omics · study · 2026
An optimized protocol for efficient derivation of pancreatic islets from multiple human pluripotent stem cell lines
Listed in NCBI GEO
Future success of cell therapy for type 1 diabetes (T1D) relies on robust protocols for the differentiation of stem cells to pancreatic islets.
Description
However, currently established protocols generate stem cell-derived pancreatic islets (SC-islets) which contain non-endocrine cell populations and have immature functionality. Herein we report the development of a new protocol for the generation of potent SC-islets enriched in functional endocrine cells from multiple human pluripotent stem cell (hPSC) lines.
We show that laminin-521 efficiently supports monolayer differentiation of 8 testes hPSC lines to endocrine progenitor (EP) stage. The efficiency was further improved by reducing the pancreatic progenitor (PP) stage. Importantly, we find that aggregation of EP reduces unwanted non-endocrine and proliferative cells.
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Subsequent suspension cultures generate SC-islets with strong in vitro glucose responsiveness, similar to human islets. Upon transplantation of 300 SC-islets into the anterior chamber of the eye of a diabetic mouse model, the SC-islets undergo further maturation in vivo and re-establish normal glycemic control. The SC-islets before and after transplantation are to a major extent free of non-endocrine cell populations.
In conclusion, our robust differentiation protocol results in endocrine enriched and highly functional SC-islets that should be well suited for transplantation into patients with T1D.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE270nnn/GSE270864 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE270864 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1128662 ncbi.nlm.nih.gov/bioproject/PRJNA1128662 ↗
project · from NCBI GEO
- PubMed 41997152 pubmed.ncbi.nlm.nih.gov/41997152 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE270864 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |