Data · dataset · 2023
Single-Cell Transcriptomics Analysis of Cardiac Fibroblasts from the Collagen1a2 Lineage Reveals that They Show Endothelial Features
Listed in Teesside University Research Data Repository
Cardiac fibroblasts (CFs) are a distinct cell type of mesenchymal origin with highly heterogeneous.
Description
A subset of CFs undergoing mesenchymal-endothelial transition (MEndoT) and contributes to cardiac repair by revascularizing the damaged myocardium. We used Col1a2CreERT:R26RtdTomato transgenic mice for tracking resident CFs (RCFs) and 10x scRNA-Seq analysis to determine the subgroup of pre-existing CFs involved in MEndoT that respond to transverse aortic constriction (TAC) injury.
The cell trajectory assay showed the presence of cluster 1 RCFs showed the phenotype of stem cells, indicates that these clusters are diverse with regard to differentiation and directly contribute to angiogenesis. Sorted CD200+ CFs showed markedly higher expression levels of pro-angiogenesis-related genes and tube formation ability. Our findings indicated that CD200+ cells are the main group of CFs involved in MEndoT and plays a major role in cell differentiation.
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Our study will aid the development of new CF-targeted therapeutic strategies for treating cardiac hypertrophy.
Links
Where it is published
- DOI doi.org/10.17632/gmbnmrcsbd.1 ↗
DOI / persistent id · from researchdata tees ac uk
Catalogue records · 1
- OAI-PMH record data.mendeley.com/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Adata… ↗
metadata API · from researchdata tees ac uk
Topics
- From keywords
- Earth & Environmental Science
- Inferred from text
- Heart 65% · Single-cell RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Teesside University Research Data Repository | oai:data.mendeley.com/gmbnmrcsbd.1 | 5 h ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[anatomy].local:anatomy:heart | enrichment · researchdata tees ac uk | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[field].local:field:earth-environmental | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · researchdata tees ac uk | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| title | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/title |