Omics · study · 2026
Transcriptomic determinants of humoral immunity for heterogeneously antigenic PL45 cells
Listed in NCBI GEO
Ascitic fluid from patients with pancreatic ductal adenocarcinoma (PDAC) contains IgG antibodies that bind to allogeneic PDAC cell lines, including the commercially available PDAC cell line PL45.
Description
PL45 cells exhibited heterogeneous binding by IgG from patients with PDAC (A1 and A14). We hypothesized that transcriptomic heterogeneity within the cell line contributes to the observed antigenic heterogeneity.
To investigate potential transcriptomic determinants of humoral antigenicity, PL45 cells were stained with ascitic IgG from patients A1 or A14 and flow-cytometrically sorted using a fluorescently labeled anti-human IgG detection antibody. RNA was isolated from the IgG-positive and IgG-negative PL45 subpopulations and subjected to bulk RNA sequencing followed by DESeq2 differential expression analysis.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE334nnn/GSE334534 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE334534 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1475408 ncbi.nlm.nih.gov/bioproject/PRJNA1475408 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE334534 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |