Effect of TENT2 knockdown on miRNA profiling in human lung endothelial cells

The mature miRNA is then loaded onto an argonaute (Ago) protein to form an RNA-induced silencing complex (RISC) and target messenger RNAs. Most of the mechanisms of miRNA regulation target one of the steps in the biogenesis.

However, through analysis of miR-1 precursors, we demonstrate that VEGF stimulation induces degradation (decay) of mature miR-1-3p without affecting its precursors. This analysis also identified a specific miRNA 3’-adenylation pathway that targets the guide strand after RISC loading. VEGF induced the expression of terminal nucleotidyl transferase 2 (TENT2, also known as PAPD4 or GLD2), one of the main enzymes that mediate 3’ miRNA adenylation, and knockdown of TENT2 inhibited miR-1 decay.