Omics · study · 2026
Transcriptomic analysis of HEK-293 cells subjected to prolonged mild acidification (pH 6.6) and treatment with an SR protein kinase inhibitor (SRPIN340)
Listed in NCBI GEO
Acidosis, the hallmark of aggressive cancer and chronic inflammation, is associated with altered transcription and alternative splicing.
Description
These alterations may be due to the repressed release of transcription and splicing factors from splicing speckles, which are scaffolded by phosphorylated SR-rich proteins. We aimed to partially verify this hypothesis and clarify whether changes in SR solubility caused by acidification and dephosphorylation have any common effects on transcription and alternative splicing.
For this, we modelled prolonged (2 d) non-damaging acidosis in non-synchronized HEK-293 cells and compared the transcriptomic changes to those induced by a subtoxic concentration of a specific SR protein kinase inhibitor SRPIN340.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328432 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328432 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1454972 ncbi.nlm.nih.gov/bioproject/PRJNA1454972 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE328432 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |