Supplementary file 1_Secretory carrier membrane protein 3 modulates nuclear ERK1/2 signaling and drug response in triple-negative breast cancer.zip

Identifying modulators that shape ERK1/2 output and influence responses to ERK1/2 inhibitors is therefore needed. The endosomal trafficking protein secretory carrier membrane protein 3 (SCAMP3) is a positive regulator of epidermal growth factor receptor (EGFR) signaling and ERK1/2-dependent phosphoproteomics programs in TNBC.

However, the mechanism by which SCAMP3 modulates ERK1/2 signaling and targeted drug response remains unclear. </p>Methods<p>Here, we combined SCAMP3 knockout with pharmacological ERK1/2 inhibition to evaluate SCAMP3's contributions to MAPK signaling, ERK1/2 distribution, cell-cycle control, apoptosis, and in vivo drug response. </p>Results<p>We found that SCAMP3 expression is upregulated in TNBC models harboring intrinsic MAPK-activating alterations compared with MAPK wild-type breast cancer cells.