Omics · study · 2026
RNA sequencing of polysome-associated transcripts in EIF4A1-overexpression and mock-virus APOE4 NGN2 neurons
Listed in NCBI GEO
Polysome-associated RNA was profiled in human NGN2-induced neurons of APOE4 genotype after mock-virus treatment or EIF4A1 overexpression.
Description
Neurons were co-plated with rat astrocytes, harvested at 7 weeks post-differentiation, and RNA was isolated from pooled heavy polysome fractions (fractions 17, 18, and 19) following sucrose-gradient fractionation. Libraries were prepared after poly(A) enrichment using SMART-Seq v4 with tagmentation and sequenced as 150-bp paired-end reads on an Illumina NovaSeq X Plus platform.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE324nnn/GSE324565 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE324565 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1435814 ncbi.nlm.nih.gov/bioproject/PRJNA1435814 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE324565 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |